SegDesign enables targeted local protein structure remodeling in enzymes, suggesting a breakthrough in protein optimization.
Key Points
The aim is to develop a controllable framework for segment-level protein engineering that allows precise modifications while preserving the overall structure.
Introduced SegDesign as a modular framework for segment-level engineering.
Integrated processes including backbone reconstruction and sequence redesign.
Implemented multi-stage structural evaluation for optimized designs.
Successfully converted a flexible loop in Gallus gallus TdT into a stable α-helix or β-strand.
Demonstrated preserved global protein fold during structural modifications.
Validated framework across six additional enzymes, highlighting versatility.