Determination of Relative Stereochemistry of an Unknown Carbohydrate

Key Points

• This research aims to establish the relative stereochemistry of an unknown carbohydrate through derivatization and spectroscopic techniques.
• An unknown carbohydrate was derivatized to form a single diastereomer.
• The diastereomer was recrystallized for purification.
• Correlation spectroscopy (COSY) was used to assign hydrogen peaks.
• 3JHH coupling constants were analyzed based on the Karplus relationship.
• A single diastereomer was successfully obtained from the unknown carbohydrate.
• Hydrogen peaks were assigned accurately using COSY spectroscopy.
• The relative stereochemistry was assigned based on 3JHH coupling constants.