This study presents a comparative LC-HRMS-based phytochemical profiling of Campanula saxifraga subsp. meyeriana (Rupr.) Ogan and Campanula saxifraga subsp. aucheri (A.DC.) Ogan collected in Azerbaijan. In C. saxifraga subsp. meyeriana, 21 compounds were identified in root extracts and 23 in aerial parts, with astragalin (672 ng/mg extract), quercetin-3-rutinoside-7-glucoside (671.9 ng/mg extract), and kaempferol (255 ng/mg extract) as major constituents. In C. saxifraga subsp. aucheri, 40 compounds were identified in roots and 25 in aerial parts, where quinic acid (3146 ng/mg extract), astragalin (1178 ng/mg extract), and quercetin derivatives predominated. The comparative profiles revealed flavonoid-rich fingerprints in meyeriana and phenolic-acid-dominated composition in aucheri, supporting chemotaxonomic differentiation. The LC-HRMS method showed good analytical performance (R2 > 0.98) and was suitable for quantitative assessment of phenolics and flavonoids in Campanula extracts. This study provides the first comprehensive LC-HRMS-based phytochemical comparison of these Caucasian subspecies and suggests potential pharmacological relevance.
Safarova et al. (Wed,) studied this question.