Photocatalytic Depletion of GSH/NADH and O 2 -Adaptive Pathway Switching in Producing ROS: Overcoming Treatment Resistances of Cancer Cells to Photodynamic Therapy and Inducing Ferroptotic Cell Death
This research develops an enzyme/light dual-activatable photosensitizer that induces ferroptosis in cancer cells, suggesting a new way to enhance the effects of photodynamic therapy.
Key Points
The aim is to develop a photosensitizer that overcomes treatment resistance in cancer cells to enhance photodynamic therapy.
Developed a selenium-based dual-activatable photosensitizer.
Employed enzymatic cleavage by aminopeptidase N to activate the prodrug in cancer cells.
Utilized light activation for further transformation into an active photosensitizer.
Assessed dual photocatalytic activities under varying oxygen conditions for ROS production.
The active photosensitizer effectively depleted glutathione and NADH levels in cancer cells.
Showed dual pathway switching in producing reactive oxygen species based on oxygen availability.
Induced significant ferroptotic cell death in cancer cells, almost exclusively.