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March 29, 20260 citationsOpen Access

An Invitro Anti-Oxidant Activity of Gentisin – An Active Fraction From Gentiana Lutea

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FHFarija K. H.HMHiba Nowrin C. M.RARumana Fathima A.

Key Points

  • The central aim is to evaluate the antioxidant activity and cytotoxicity of gentisin from Gentiana lutea.
  • In vitro assays to assess antioxidant potential
  • DPPH radical scavenging assay
  • Nitric oxide and hydroxyl radical scavenging assays
  • Hydrogen peroxide scavenging assay
  • MTT assay for cell viability assessment
  • Gentisin efficiently scavenges free radicals
  • Demonstrated cytoprotective effects against oxidative stress
  • Supported potential as a natural antioxidant
  • Effective in protecting HepG2 cells from damage

Abstract

This study evaluates the in vitro antioxidant activity of gentisin, a bioactive xanthone derivative isolated from Gentiana lutea. The antioxidant potential of gentisin was assessed through several assays: DPPH radical scavenging, nitric oxide and hydroxyl radical scavenging, hydrogen peroxide scavenging, and total antioxidant capacity. Additionally, the MTT assay was conducted to assess cell viability and cytotoxicity in HepG2 cells. The results indicate that gentisin effectively scavenges free radicals and protects cells from oxidative stress-induced damage, supporting its potential as a natural antioxidant and hepatoprotective agent.

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Cite This Study

H. et al. (2026) studied this question.

synapsesocial.com/papers/69c8c30dde0f0f753b39da7chttps://doi.org/10.5281/zenodo.19247375
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