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March 29, 2026Прикладная биохимия и микробиология / Applied Biochemistry and Microbiology

Low Temperatures Stimulate Alternative Splicing of the CPK26 Gene in Vitis amurensis Grapes

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Authors

KKK. V. KiselevADA. S. DubrovinaZOZ. V. Ogneva

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Overview

Investigates the effects of low temperatures on alternative splicing of VaCPK26 in Vitis amurensis, highlighting its role in stress resistance.

Key Points

  • To explore how low temperatures influence alternative splicing of the VaCPK26 gene in Vitis amurensis and its role in stress resistance.
  • Examined VaCPK26 transcription levels in grape leaves under various environmental factors
  • Identified full-length and short-spliced VaCPK26 transcripts
  • Conducted assays on recombinant VaCPK26 to assess its effect on salt and drought resistance
  • Under low temperature, a short-spliced VaCPK26s1 transcript was produced that lacks the 2nd exon
  • Recombinant VaCPK26 increased grape cell resistance to salt stress and drought
  • Overexpression of VaCPK26s1 had no effect on stress resistance, indicating a loss of functionality compared to full-length VaCPK26

Cite This Study

Kiselev et al. (2025) studied this question.

synapsesocial.com/papers/69c8c336de0f0f753b39de65https://doi.org/10.7868/s3034574x25050081
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