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Synapse
March 29, 2026Veterinary SciencesOpen Access

Successful Endoscope-Assisted Transcervical Insemination (TCI) in Dogs Using Sperm Recovered from Epididymides Stored at 5 °C for 24 h After Castration Prior to Semen Collection and Cryopreservation

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Authors

MBMónika BacsaESEszter SzilágyiKEKristian Erdei

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Overview

Evaluates endoscopic insemination success using cryopreserved sperm in dogs, suggesting a non-surgical alternative.

Key Points

  • The aim is to determine if sperm from refrigerated epididymides retains fertilizing ability after cryopreservation for use in non-surgical insemination.
  • Stored epididymides at 4–5 °C for 24 h after castration
  • Recovered spermatozoa via single-incision aspiration
  • Frozen using the Uppsala method and stored in liquid nitrogen
  • Inseminated a female dog in heat with thawed sperm through transcervical endoscope guidance
  • Confirmed pregnancy through ultrasonography
  • Delivered nine healthy puppies at term
  • Achieved insemination with 62 × 10^6 progressive motile spermatozoa
  • Demonstrated that 24 h refrigeration does not impair fertilizing ability of sperm

Cite This Study

Bacsa et al. (2026) studied this question.

synapsesocial.com/papers/69c8c384de0f0f753b39e649https://doi.org/10.3390/vetsci13040326
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Also Consider

Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1A comparative study of canine epididymal sperm collection techniques and cryopreservation2023 · 5 citations
  2. 2Unilateral Intrauterine Insemination with Cryopreserved Caudal Epididymal Sperm Recovered from Refrigerated Canine Epididymides2005 · 29 citations