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March 29, 2026Радиационная биология Радиоэкология / Radiation biology Radioecology0 citations

Experimental evaluation of hypobiosis as a method of increasing the bodys resistance in modeling acute radiation sickness in rats

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AMA. F. MakarovMKM. A. KotskyATA. A. Tonshin

Key Points

  • This research aims to test if artificial hypobiosis can improve survival rates in rats exposed to ionizing radiation.
  • Divided male rats into experimental and control groups.
  • Induced hypobiosis in the experimental group via intramuscular alpha-methyldopa injection.
  • Exposed both groups to acute radiation using cobalt-60 gamma-ray sources.
  • Monitored rats' body weights and recorded the timing of weight loss cessation and weight gain initiation.
  • Mortality of 50% of hypobiosis-treated rats occurred 1.4 times later than the control group.
  • Approximately 50% of the hypobiosis group survived a lethal dose of 9.5 Gy, while the control group had 100% mortality.
  • Weight gain began in surviving rats on the 18th day of observation.

Abstract

To verify the efficacy of artificial hypobiosis as a method for the preventive protection of living organisms against ionizing radiation, the experimental study was conducted. The study involved male rats, which were divided into two groups: the experimental group and the control group. Rats in the experimental group underwent induction of artificial hypobiosis through intramuscular injection of the alpha-methyldopa (CAS: 555-30-6). Both groups of rats underwent simulated acute radiation exposure. The radiation was delivered using a “GUT-200M” gamma-ray irradiator, powered by “GIK-7-4” cobalt-60 sources. The absorbed dose administered was 9.5 ± 0.1 Gray (Gy). Afterward, the rats body weights were monitored daily, and the timing of weight loss cessation and weight gain initiation, as well as death, were recorded. The findings of the study revealed that the mortality of 50% of the laboratory rats subjected to artificial hypobiosis occurred 1.4 times later compared to the control group.; Artificial hypobiosis has been shown to prevent the death of approximately 50% of animals exposed to a lethal dose of ionizing radiation equivalent to 9.5 Gy (100% mortality in the control group). In surviving animals weight gain has been observed beginning on the 18th day of the study.

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Cite This Study

Makarov et al. (2025) studied this question.

synapsesocial.com/papers/69c8c384de0f0f753b39e67chttps://doi.org/10.7868/s3034589825060054
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