Control Levels of Translocations in Human Peripherial Blood Lymphocyte Cultures Determined by Multicolor Fish Staining

Introduction. In retrospective dose assessment using multicolor FISH staining, adequate dose assessment requires information on the average population levels of translocations in the control. Materials and Methods. Using the multi(24) color FISH method, chromosome aberrations were analyzed in peripheral blood lymphocyte cultures from 32 relatively healthy volunteer donors aged 21 to 72 years. Results. From 98 to 473 metaphases were analyzed in individual donors. The main quantity of FISH-registered chromosomal aberrations were reciprocal translocations with a frequency (per 100 cells) of 0.758 ± 0.118, which was significantly 5.9 times higher than the level of all unstable aberrations. The average frequencies of recorded translocations were 0.77 ± 0.13 and 0.74 ± 0.23 for women and men, respectively. Conclusion. In the control group, the observed frequencies of translocations in peripheral blood lymphocyte cultures did not have gender differences. Also, no significant effect of various types of medical diagnostic radiation, COVID-19 disease, and smoking on this cytogenetic indicator was found. A moderate correlation was observed between the frequencies of FISH translocations and the age of the donors.