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March 31, 2026ChemFoodChem0 citations

Design of a Fluorescent Boronic Acid‐Functionalized Pyranoflavylium Receptor to Host and Release Diol‐Rich Bioactive Analytes in a pH‐Dependent Manner

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BSBeatriz P. SenraMCMariana CunhaAPAna Sofia Pires

Key Points

  • The research aims to create a fluorescent receptor that can selectively host and release bioactive diol-rich analytes in response to pH changes.
  • Synthesis of a pyranoflavylium-boronic acid derivative
  • Characterization of the receptor and its complexation with diol-based analytes
  • Fluorescence technique analysis for binding interactions and pH dependence
  • 11B NMR spectroscopy to confirm complex formation
  • (+)‐Catechin showed the highest affinity with the dye, with an association constant of 48895 ± 1 M −1.
  • L-lactic acid and D-glucose had lower binding constants, indicating weaker interactions.
  • Covalent interactions are pH-dependent, promoting analyte release in acidic conditions.

Abstract

ABSTRACT Smart materials composed of bioactive compounds and stimuli‐responsive dyes are currently of great interest in different disciplines, from the food industry to biomedical applications in sensing, theranostics, imaging, and drug/gene delivery. This work synthesized a new symmetrical pyranoflavylium‐boronic acid derivative, structurally characterized and complexed with relevant diol‐based biological molecules such as (+)‐catechin, L‐lactic acid, and D‐glucose. The model systems were studied by fluorescence technique in the presence and absence of those ligands and were able to recognize those analytes with different association constants and in a pH‐controlled fashion. Overall, (+)‐catechin revealed the greatest affinity with the dye with an association binding constant ( K a ) of 48895 ± 1 M −1 rather than with L‐lactic acid ( K a 595 ± 2 M −1 ) or D‐glucose ( K a 7 ± 2 M −1 ). The covalent interaction between (+)‐catechin and the pyranoflavylium receptor was demonstrated to be pH‐dependent, occurring near of the dye p K a (pH∼8), while for acidic pH values the (+)‐catechin is released from the dye, recovering the initial fluorescence of its free flavylium cation species. The formation of the diol‐boronate ester complex was confirmed by 11 B NMR spectroscopy in the presence of (+)‐catechin only at a neutral pH value.

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Cite This Study

Senra et al. (2026) studied this question.

synapsesocial.com/papers/69cb64f0e6a8c024954b8f35https://doi.org/10.1002/cfch.70014
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