What question did this study set out to answer?

The study aims to genetically differentiate between Fasciola hepatica and Fasciola gigantica using 28S rDNA analysis.

April 1, 2026Open Access

Sequencing strain Fasciola species in Cattle in Iraq

Puntos clave

The study aims to genetically differentiate between Fasciola hepatica and Fasciola gigantica using 28S rDNA analysis.
Isolated adult Fasciola worms from the liver of cattle
Extracted genomic DNA using a specific kit
Used 21 bp specific primers targeting the 28S rDNA gene
Conducted BLAST and PCR analysis for phylogenetic variation
Showed approximately 80% genetic similarity between the two Fasciola species
Revealed significant species-specific conservation and polymorphism
Indicated possible strain hybridization within the species

Resumen

Fascioliasis is a significant zoonotic disease caused by Fasciola species that affects the human and animals like sheep, cows and buffaloes worldwide that causes public and veterinary health problems. The aim of this study focusing on the genetic differentiation between the two species of Fasciola (F. hepatica and F. gigantica) by using large subunit of 28S rDNA gene. A total of 45 adult worms were isolated from liver of different cattle in Iraq-Basrah Provence, extraction of genomic DNA using special kit was performed to isolated Fasciola species with 21 bp specific primers included that target 28S rDNA. The BLAST and PCR products showed evolutionary phylogenetic and variation genetically with similarity in about 80% between the two species of Fasciola. The results phylogenetically concluded a highly species specific conservation and polymorphism with possible strain hybridization

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