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April 1, 2026Plasma Processes and Polymers0 citationsOpen Access

Low‐Temperature Atmospheric‐Pressure Plasma Assisted Therapy for Onychomycosis

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HCHua‐Lin ChenYCY L ChengJWJong‐Shinn Wu

Key Points

  • To evaluate the effectiveness of argon low-temperature plasma combined with medication for treating onychomycosis.
  • Characterization of low-temperature atmospheric-pressure plasma jet (APPJ) operation.
  • Cultivation of Candida albicans on bovine hoof models for infection assessment.
  • Comparison of combined plasma and medication therapies against medication alone.
  • Measurement of yeast colony count reduction after plasma exposure.
  • Assessment of drug penetration depth through infected specimens.
  • APPJ exposure achieved a 1-log reduction in yeast colonies within 2 minutes.
  • Combined plasma and medication therapy significantly reduced yeast burden in infected samples (p ≤ 0.05).
  • Superior antimicrobial efficacy observed in mildly infected specimens with combined therapy (p ≤ 0.01).
  • Plasma-assisted treatment demonstrated complete drug penetration to approximately 200 µm depth.

Abstract

ABSTRACT This study evaluates an argon low‐temperature atmospheric‐pressure plasma jet (APPJ) combined with medication for the treatment of onychomycosis. Plasma characterization confirmed operation below 25°C, indicating biomedical suitability. Using Candida albicans cultured on bovine hoof models, APPJ exposure achieved an approximately 1‐log reduction in colony counts within 2 min. For embedded infections, plasma treatment, medication alone, and combined therapies were compared. The combined plasma–medication approach significantly reduced yeast burden in moderately infected samples ( p ≤ 0.05) and showed superior antimicrobial efficacy in mildly infected specimens compared with medication alone ( p ≤ 0.01). Plasma‐assisted drug permeation experiments further demonstrated complete penetration through the specimen, reaching the bottom at approximately 200 µm depth.

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Cite This Study

Chen et al. (2026) studied this question.

synapsesocial.com/papers/69cd7b065652765b073a8b3chttps://doi.org/10.1002/ppap.70162
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