The water-soluble extract of Adonis mongolica increased heart rate by 32 beats/min and mean blood pressure by 23 mmHg in vivo, and increased atrial contractile force by 112% in vitro.
The water-soluble extract of Adonis mongolica exerts positive inotropic and vasopressor effects mediated by increased sympathetic tone and contains multiple cardiac glycosides, providing a pharmacological basis for its traditional use in heart failure.
valor p: p=<0.01
Background: Adonis mongolica (Ranunculaceae) is one of the endemic plants in Mongolia and has been used as a medicinal herb in Mongolian traditional medicine to treat patients with congestive heart failure showing tachycardia and edema. Although the plant has been empirically used in the last three decades, the precise information regarding its cardiovascular profile is still limited. Methods: We assessed the cardiohemodynamic and electrophysiological profile of the water-soluble extract of A. mongolica using the guinea pig in vivo model (n = 4) and in vitro preparation (n = 5 - 17). In addition, the onset mechanism of the extract-induced effects on the heart rate and blood pressure in vivo (n = 4), and the atrial rate and contractile force in vitro (n = 4 - 5) were pharmacologically analyzed. Results: The extract exerted the positive chronotropic, negative dromotropic, and vasopressor effects in addition to the proarrhythmic action in vivo. Meanwhile, it modestly decreased the atrial rate and aortic tension but increased the atrial contractile force in vitro. The pretreatment of dl-propranolol and prazosin significantly suppressed the positive chronotropic and vasopressor actions induced by the extract in vivo, indicating that the extract increased the sympathetic tone. Also, liquid chromatography-mass spectrometry analysis showed that water-soluble extract of A. mongolica contained eight kinds of cardiac glycosides. Conclusions: These results indicate that cardiac glycosides in the water-soluble extract of A. mongolica may explain currently observed various cardiovascular effects.
Hiroko et al. (Fri,) conducted a other in Healthy (Animal Study) (n=14). Adonis mongolica water-soluble extract vs. Pre-drug baseline was evaluated on Change in heart rate and mean blood pressure in vivo (p=<0.01). The water-soluble extract of Adonis mongolica increased heart rate by 32 beats/min and mean blood pressure by 23 mmHg in vivo, and increased atrial contractile force by 112% in vitro.