Abstract 7673: 9-channel spatial co-detection of clinically relevant protein and RNA targets in FFPE tumors with HCR™ Gold and MoxiePlex.

Key Points

• The aim is to assess a new method for simultaneous detection of protein and RNA in fixed tumor samples, overcoming limitations of existing techniques.
• Used HCR™ Gold IF for protein detection and HCR™ Gold RNA-FISH for RNA detection.
• Tested 7-plex and 9-plex configurations on the MoxiePlex imaging platform.
• Generated single-stain reference slides for exposure and spectral bleed-through settings.
• Performed whole-slide imaging in a single acquisition round with automated spectral unmixing.
• Achieved bright signals while preserving tissue architecture.
• Localized protein and RNA targets correctly within tumor and stroma regions.
• Demonstrated consistent imaging performance suitable for routine multiplex scanning.

Abstract