Abstract ADCs are typically synthesized one by one and not amenable to early screening using a platform method. We developed a plate-based conjugation platform that integrates early screening with in vitro characterization using maleimide-based linker-payloads. With a standardized, assay-compatible protocol, this high-throughput system rapidly assesses DAR, purity, yield, cytotoxicity, and binding. It supports antibody screening with fixed linker-payloads, payload screening with fixed antibodies, and ADC characterization under varied conditions, providing a powerful tool for rational ADC design. Citation Format: Zeinab Fereshteh, Madhavi Latha S. Chalasani, Rupa Sarkar, Julien Dugal-Tessier, Nareshkumar Jain, . Plate-based conjugation for antibody and payload optimization in ADC development abstract. In: Proceedings of the American Association for Cancer Research Annual Meeting 2026; Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 1679.
Fereshteh et al. (Fri,) studied this question.