This abstract demonstrates the impact of transmissible ER stress in altering immune responses in PDAC, suggesting critical immune interactions within the tumor environment.
Key Points
To investigate the impact of transmissible ER stress from PDAC cells on the immune profile of bone marrow-derived macrophages (BMDMs).
Utilized murine Panc02 and KPC cells as transmitter cells treated with thapsigargin (Tg).
Cultured murine BMDMs in ER stress-conditioned medium from treated tumor cells.
Analyzed transcriptional changes in BMDMs, focusing on proinflammatory and immunosuppressive markers.
BMDMs showed significant upregulation of proinflammatory modulators like iNOS and NF-κB.
Expression of Arg1 indicated an immunosuppressive macrophage phenotype.
The findings reflect a hybrid state of inflammation and immune regulation in the PDAC tumor microenvironment.