Abstract 7668: Characterization of outer membrane vesicles (OMVs) from pks+ NC101 and pks- DH10B E. coli strands.

Puntos clave

• The aim is to investigate the mechanism of colibactin export in pks+ E. coli via outer membrane vesicles (OMVs).
• Isolated OMVs from pks+ NC101 and pks- DH10B E. coli strains using exoEasy Maxi Kit.
• Quantified OMVs with a BSA-based protein assay.
• Analyzed OMV stability and morphology using dynamic light scattering (DLS).
• Examined metabolites via mass spectrometry to confirm the presence of colibactin-related intermediates.
• Performed proteomic analysis of OMVs to identify proteins involved in host interaction.
• OMV preparations had concentrations consistent with typical outputs.
• Dynamic light scattering showed stability and specific morphological characteristics of extracted OMVs.
• Mass spectrometry confirmed the presence of colibactin-related intermediates in OMVs.
• Proteomic analysis identified proteins important for OMV-host interactions.