Brucellosis is a globally prevalent zoonosis that causes abortion and infertility in livestock, leading to substantial economic losses. Sensitive and reliable quantification of Brucella antibodies, particularly at trace levels, is critical for early diagnosis. In this work, an electrochemical immunosensor was developed by integrating electric field-assisted antigen immobilization with an electrode platform. The electrode was first electrochemically pretreated to improve interfacial reproducibility, and then sequentially modified with L-cysteine and glutaraldehyde to construct an antigen-coupling layer. During antigen immobilization, a custom-built electric field device was applied to regulate the interfacial arrangement of Brucella antigens. The fabrication process was characterized by scanning electron microscopy and cyclic voltammetry, and the analytical performance was evaluated by electrochemical impedance spectroscopy and voltammetric measurements. Under the optimized conditions, the proposed immunosensor exhibited a linear response to Brucella antibodies over the range of 1 × 10−6–10 IU/mL, with a correlation coefficient of 0.99 and a detection limit of 2.04 × 10−7 IU/mL. The sensor also showed acceptable specificity, repeatability, and short-term storage stability, with recoveries of 93.15–99.14% in spiked milk samples. These results indicate that electric field-assisted immobilization can serve as a useful interfacial regulation strategy for Brucella immunosensing and support the analytical feasibility of the proposed platform under controlled experimental conditions. Further validation in more complex biological matrices is still required.
Yang et al. (Fri,) studied this question.
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