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April 8, 2026PLoS ONEOpen Access

Association between chronic stress and the epigenome: Exploration of psychological and biological stress

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Authors

MMMelissa A. MillerRBRobin D. BrownCKCole Krautkramer

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Overview

Longitudinal exploration reveals distinct epigenetic associations with psychological and biological stress, suggesting complex interactions.

Key Points

  • The study aims to explore the distinct epigenetic effects of psychological and biological stress on health outcomes.
  • Conducted a longitudinal design over 9 weeks with 69 healthy participants aged 18–35.
  • Assessed self-reported psychological stress multiple times each week.
  • Measured hair cortisol concentration and genome-wide DNA methylation at the end of the study.
  • Analyzed associations between psychological stress, cortisol levels, and DNA methylation at multiple CpG sites.
  • Identified 956 CpG sites linked to chronic psychological stress and 10,335 CpG sites linked to hair cortisol concentration.
  • Elevated psychological stress was generally associated with DNA hypomethylation, while elevated hair cortisol concentration correlated with DNA hypermethylation.
  • Self-report stress and hair cortisol concentration showed no correlation.
  • Certain CpG sites showed opposing methylation patterns for psychological stress and cortisol, indicating competition in biological pathways.

Cite This Study

Miller et al. (2026) studied this question.

synapsesocial.com/papers/69d5f14b74eaea4b11a7ae03https://doi.org/10.1371/journal.pone.0346517
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