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April 8, 2026Bioelectricity

Rapid Hyperpolarization of the Cellular Membrane Potential Co-Occurs with Slowed Bacterial Elongation

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Authors

MHMorgan HeckmanSRSigrid N. Real-Aguilar

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Overview

Demonstrates the link between membrane potential and bacterial elongation in Bacillus subtilis, suggesting implications for cell growth under stress.

Key Points

  • This research aims to explore the relationship between membrane potential spikes and bacterial elongation in Bacillus subtilis, particularly under ethanol stress.
  • Measured membrane potential using tetramethyl rhodamine methyl ester, a fluorescent dye.
  • Simultaneously recorded cell fluorescence and elongation using fluorescence and bright field microscopy over 30 minutes.
  • Analyzed images with ImageJ and custom MATLAB software.
  • Cells that experience rapid spikes in membrane potential exhibit slower elongation rates than those without spikes.
  • This pattern is consistent in both untreated and ethanol-treated B. subtilis cells during the observation period.

Cite This Study

Heckman et al. (2026) studied this question.

synapsesocial.com/papers/69d5f14b74eaea4b11a7aea5https://doi.org/10.1177/25763113261440403
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