Virtual screening and cellular validation of dolutegravir as a BRD9 inhibitor for attenuating pyroptosis in peritoneal mesothelial cells

Key Points

• The research aims to investigate dolutegravir's role as a BRD9 inhibitor and its effects on pyroptosis in mesothelial cells.
• Conducted virtual screening to identify dolutegravir as a BRD9 inhibitor.
• Examined the impact of dolutegravir on high glucose-induced pyroptosis.
• Studied the modulation of NLRP3 mRNA stability in mesothelial cells.
• Confirmed dolutegravir as a novel BRD9 inhibitor.
• Demonstrated reduced pyroptosis in mesothelial cells upon dolutegravir treatment.
• Showed that BRD9 regulates pro-fibrotic signaling related to NLRP3 mRNA stability.

Abstract