Age-associated DNA methylation loci at lncRNA genomic regions revealed by Oxford Nanopore whole-genome sequencing using four methylation callers

Key Points

• This research aims to identify DNA methylation changes associated with aging using advanced sequencing techniques.
• Utilized Oxford Nanopore whole-genome sequencing
• Applied four methylation callers for analysis
• Focused on lncRNA genomic regions
• Compared findings with existing bisulphite methods
• Identified specific DNA methylation loci linked to age
• Enhanced accuracy of age inference from compromised samples
• Demonstrated effectiveness of Oxford Nanopore sequencing over traditional methods

Abstract