A Dual Cell‐Line Molecular Platform to Assess Sub‐Threshold Biocompatibility: Application to Processed Orthodontic Clears Aligners

THP-1 and MCF-7 pair is a bioactivity detection sub-threshold system that is both efficient and sensitive as well as a highly effective preclinical test for dental material. The findings of this study confirm that orthodontic clear aligners, even after being trimmed on 3D-printed models, do not exhibit antigenic properties. This supports their continued use in intraoral applications where the absence of immunological reactivity is essential during the development phase.