From cysteines to histidines - chemically distinct Cu(II)-binding motifs in the C-terminal region of archaeal CopT regulators

Key Points

• This research aims to explore the metal-binding properties of CopT regulators in archaea, specifically focusing on their C-terminal regions.
• Chemical analysis of Cu(II)-binding motifs
• Characterization of metal-binding properties in CopT proteins
• Comparative studies of binding affinities
• Identified distinct Cu(II)-binding motifs involving cysteines and histidines
• Demonstrated variability in metal-binding capabilities among different CopT regulators
• Highlighted the importance of these motifs in archaeal metal homeostasis.

Abstract