Fabrication of methotrexate conjugated multi-walled carbon nanotubes for the evaluation of cytotoxic potential at biochemical and molecular level modulating BAX , BCL-2 and telomerase expression

Key Points

• The aim is to evaluate the cytotoxic effects of methotrexate-conjugated multi-walled carbon nanotubes at the biochemical and molecular levels.
• Fabricated methotrexate-multi-walled carbon nanotube nanoconjugate.
• Characterized the nanoconjugate for various properties.
• Conducted in vitro assays to assess cytotoxic activity, hemocompatibility, and anti-angiogenic effects.
• The MTX-MWCNT nanoconjugate exhibited significant cytotoxic activity.
• Altered expression levels of BAX and BCL-2 were observed.
• Demonstrated potential for anti-angiogenic effects through morphological alterations.

Abstract