Gordonia abscondita sp. nov. and Pseudochrobactrum mosebachae sp. nov., two bacterial species isolated from the burying beetle Nicrophorus vespilloides

Two novel bacterial strains, designated MP11MiT and MP213FoT, were isolated from the burying beetle Nicrophorus vespilloides and subjected to a polyphasic taxonomic study. Strain MP11MiT was placed in the genus Gordonia, showing the highest 16S rRNA gene sequence similarities to Gordonia liuliyuniae HY366T (98.8% sequence identity), followed by Gordonia zhenghanii HY002T (98.4%). The strain exhibited low genomic relatedness to its closest relatives [ANI (average nucleotide identity) T was affiliated with the genus Pseudochrobactrum, with Pseudochrobactrum algeriense LMG 32378T as its closest relative. ANI (79.4-79.9%), dDDH (22.8-23.1%), average amino acid identity (82.5-83.8%) and percentage of conserved proteins (71.6-77.2%) values confirmed its clear separation from all hitherto recognized species of this genus. The genome of MP213FoT comprises a circular chromosome and a plasmid carrying conserved genus-specific features. Analysis of specialized biosynthetic potential identified 13 gene clusters in MP11MiT and 4 in MP213FoT, highlighting their capacity for specialized metabolite biosynthesis. Based on phylogenomic, chemotaxonomic and phenotypic characteristics, strains MP11MiT and MP213FoT are proposed as representatives of two novel species, for which the names Gordonia abscondita sp. nov. (type strain MP11MiT=DSM 113667=CCM 9219) and Pseudochrobactrum mosebachae sp. nov. (type strain MP213FoT=DSM 113682=CCM 9218) are introduced.