Abstract Functional maturation of post‐extraction blood clots is the biological cornerstone for alveolar socket preservation, where fibrin‐mediated osteogenic cell recruitment and matrix mineralization dictate long‐term bone regeneration outcomes. To safeguard this critical process, we engineer an octopus‐inspired tissue‐adhesive nanofiber membrane (ANF) that actively stabilizes clots without occlusive disruption. Its bilayer architecture combines an outer hydrophobic nanofibrous layer that suppresses bacterial infiltration by 93.09 ± 0.55% and an inner charge‐heterogeneous Polyethyleneimine (PEI)/Polyacrylic acid (PAA) nanofiber network. Upon application, this system creates an adaptive tissue adhesive strength of 5.87 ± 0.57 kPa through biomimetic suction, which reaches 17.37 ± 3.26 kPa with hydrogen bonding on palatal tissues. At the same time, opposite‐charged interfaces of PEI/PAA nanofiber electrostatically enrich fibrinogen and coagulation factors. The blood clotting index was decreased to 1.43 ± 0.09%. Crucially, this non‐occlusive strategy avoids filler‐induced mechanical interference with natural clot retraction, while establishing a protective microenvironment for the re‐epithelialization of the gingiva. Rat anterior tooth extraction model confirms synergistic biological advantages: wound contraction is enhanced on days 7 and 14, respectively. ANF significantly improved alveolar ridge height reservation by 69.69 ± 2.53%, with a new bone area of 89.83 ± 2.06%. By accelerating hemostasis with unperturbed biological remodeling, this membrane transforms clot maturation into functional bone regeneration.
Ma et al. (Fri,) studied this question.