Purpose The 2015 guidelines recommend using a large, diverse, and race‐matched reference database. However, defining expectations in this context is subjective due to factors like genetic diversity and penetrance. ClinGen forms VCEPs to provide gene‐specific interpretations of ACMG/AMP guidelines, including population information. Our study evaluates VCEP guidelines on allele frequency information. Methods We analyzed genetic codes in databases to determine the frequency and potential pathogenicity of variants among 39 VCEPs, considering factors like allele frequency thresholds and disease prevalence. Results Our analysis found a variety of approved cutoffs among VCEPs, showing diverse disease mechanisms. We also noted variability in methods used to establish cutoffs and inconsistencies in parameters deemed necessary for approved thresholds. Conclusions Understanding thresholds requires knowledge of genetics and diseases. VCEP guidelines on allele frequency evidence can help curators identify recommended thresholds. However, more guidance is needed for consistency in population evidence utilization.
Wang et al. (2026) studied this question.