A Four-Step Micro-Research Closed-Loop Model via Online Spreadsheets for Molecular Biology Experimental Teaching: A Polymerase Chain Reaction (PCR) Case Study
A nonrandomized quasi-experimental study evaluates a low-cost model for improving PCR knowledge in college students, suggesting effective teaching methods.
Key Points
To develop and assess a low-cost, micro-research closed-loop teaching model for PCR experimentation.
Conducted a nonrandomized quasi-experimental study with 33 students at one institution.
Divided participants into an experimental group using an online model and a control group with traditional instruction.
Administered a 20-item PCR knowledge test and a 5-item confidence survey before and after the intervention.
Analyzed data using independent-samples t-tests and Fisher's exact test.
The experimental group completed protocol design significantly faster than the control group.
They exhibited a higher first-pass approval rate than the control group.
Lower operational error rates were recorded for the experimental group.
There was a greater increase in confidence in the experimental group.
Both groups showed significant improvements in PCR knowledge, but the experimental group had a larger knowledge gain.