Proteomic Trajectories of Metabolic and Proteostatic Adaptation During Normothermic Liver Perfusion

Key Points

• The research aims to explore metabolic and proteostatic adaptations during normothermic liver perfusion and their association with biliary outcomes.
• Conducted sequential tissue proteomics during normothermic liver perfusion (NMP)
• Analyzed metabolic and proteostatic changes in liver grafts
• Compared proteomic profiles between resilient and complicated grafts
• Resilient grafts exhibit glycolytic activation and preserved protein turnover
• Complicated grafts are characterized by increased translational and ER-stress pathways
• Findings suggest a need to refine liver viability assessment beyond traditional biochemical methods

Abstract