Microwave‐Accelerated Proteolysis Minimizes Artificial Deamidation and Enhances Speed for Peptide Mapping of Protein Pharmaceuticals

Key Points

• The aim is to reduce artificial deamidation during peptide mapping and improve efficiency in protein pharmaceuticals.
• Utilized microwave-accelerated proteolysis under optimized Tris buffer conditions
• Compared digestion times before and after microwave application
• Analyzed effects on artificial deamidation in IgG and BSA proteins
• Reduced digestion time from 16-18 hours to minutes
• Lowered artificial deamidation by approximately 60%-80%
• Findings in IgG and BSA indicate consistency and reliability for quality assessments

Abstract