Malaria remains a major global health challenge, particularly in sub-Saharan Africa where Anopheles mosquitoes transmit the Plasmodium parasites. Resistance to insecticides remains an obstacle in spite of the efforts to control malaria vector. The interaction between Plasmodium parasites and mosquito vectors, with a focus on the immunity of mosquitoes and approaches to combat malaria, is examined in this review. This review explores the potential of genetic approaches including CRISPR-Cas9, Wolbachia, RNA interference (RNAi), and symbiont-based strategies for the control of malaria vector. The innate immune system of Anopheles mosquitoes that identify, recognize, and limit Plasmodium infection through pathogen recognition receptors, signaling pathways, and effector mechanisms like antimicrobial peptides and melanization is well developed. However, Plasmodium has developed several evasion mechanisms to establish infection. This led to various genetic modification techniques being designed to reduce vector population and transmission. Gene drive such as CRISPR-Cas9 can introduce genetic alterations to interfere with the transmission of malaria; Wolbachia interferes with vector competence, RNAi-mediated gene to target relevant genes involved in reproduction and survival. Self-limiting strategies such as RIDL and pgSIT genetically modified insect releasement to the environment. mosGILT is an emerging immune regulator which has shown relevance in blocking transmission. This review explores the potential of these genetic approaches in malaria vector control efforts, highlighting their advantages and imitations. Further research should explore mosquito immune genes and pathways in developing innovative and acceptable genetic vector control approaches.
Cleanclay et al. (2026) studied this question.