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April 19, 2026INTERNATIONAL JOURNAL OF CURRENT SCIENCE0 citationsOpen Access

Comparative evaluation of chitosan based Kokum (Garcina indica), Bael (Aegle marmelos) and Jamun (Syzygium cumini) peel coatings: Impact on antioxidant, antimicrobial efficacy and protein digestibility

MAMs. Aditi AundhakarMTMr. Samarth TanpureMJMr. Piyush Jagdale

Key Points

  • This study aims to develop bioactive edible films using agricultural waste from Kokum, Bael, and Jamun peels to assess their antioxidant and antimicrobial properties.
  • Formulation of edible films using chitosan-glycerol from fruit peels.
  • Physicochemical testing for film properties such as thickness and optical characteristics.
  • Assessment of antioxidant activity using the FRAP assay.
  • In vivo antimicrobial activity testing on E. coli contaminated chicken slices.
  • In vitro protein digestibility analysis using bovine serum albumin and digestive enzymes.
  • Bael and Jamun coatings exhibited higher antioxidant activity (82.35% and better) compared to Kokum (62.75%).
  • The Bael coating achieved up to 50.5% reduction in E. coli bacteria after 5 days.
  • Digestibility results showed Bael coating at 60.20%, Kokum at 47.70%, and Jamun at 30.10%, indicating significant differences.

Abstract

This work aims to implement the principles of a circular economy in food science by utilizing agricultural waste, specifically peels of three native Indian fruits, namely Kokum (Garcinia indica), Bael (Aegle marmelos), and Jamun (Syzygium cumini), as antioxidants to formulate bioactive, edible films using chitosan-glycerol matrix. According to physicochemical tests, the prepared films had a homogeneous appearance and uniform thickness within the range of 2.55 µm to 6.55 µm. Additionally, all films had excellent optical properties due to high visible light transmittance (>80%). Moreover, the antioxidant activity, quantified based on free-radical scavenging ability measured in the FRAP assay, proved to be much better for the Bael and Jamun coatings (82.35%) than Kokum coatings (62.75%). In regard to the antimicrobial activity in vivo trials carried out on chicken slices inoculated with E.coli bacteria confirmed significant inhibition for the Bael coating with up to 30% bacteria reduction on day 3 and up to 50.5% reduction on day 5, while Jamun and Kokum coatings also exhibited steady-state inhibition at about 10%. Also an in vitro study conducted using bovine serum albumin a protein source and pepsin, pancreatin as digestive enzymes proved different levels of digestibility for the Bael coating (60.20%), Kokum coating (47.70%), and Jamun coating (30.10%). This helps to bridge the gap between waste management, microbiology, and human nutrition.

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Cite This Study

Aundhakar et al. (2026) studied this question.

synapsesocial.com/papers/69e471ef010ef96374d8e1e9https://doi.org/10.56975/ijcsp.v16i2.304243
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