Investigates gut microbiota's role in diarrhea through oxidative stress and SCFA dynamics in a mouse model, highlighting important connections.
Key Points
The study aims to explore the relationship between gut microbial composition, short-chain fatty acids metabolism, and gut-kidney oxidative stress in relation to diarrhea.
Established a mouse model of diarrhea using 20 KM mice.
Analyzed colonic microbiota composition through 16S rRNA sequencing.
Measured protein levels of SIRT3, SOD2, and ROS in colon and kidney tissues.
SIRT3 and SOD2 protein expression significantly decreased in colon and kidney tissues (P < 0.01).
SOD enzyme activity significantly reduced, with P < 0.05 in the colon and P < 0.01 in the kidney.
Increased ROS levels correlated with decreased SCFAs, especially with Streptococcus abundance.