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April 23, 2026ParasitologyOpen Access

In cold blood: Screening wild Alaskan moose ( Alces alces gigas ) for filarial nematode (Filarioidea: Onchocercidae) infections using a deep amplicon sequencing approach

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Authors

MKMatthew KulpaJCJohn A. CrouseDTDaniel P. Thompson

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Overview

Demonstrates the detection of filarial nematodes in wild Alaskan moose, suggesting effective monitoring methods.

Key Points

  • Evaluate the presence and diversity of filarial nematodes in wild Alaskan moose using advanced sequencing techniques.
  • Collected blood samples from wild moose in the Kenai Peninsula, AK.
  • Screened samples using deep amplicon sequencing targeting the cox1 gene and Modified Knott's Test.
  • Analyzed data to identify co-infections among filarial nematodes.
  • Filarial DNA detected in 51.58% of samples tested via deep amplicon sequencing.
  • Co-infections identified in 13.04% of samples using sequencing compared to 8.70% via traditional methods.
  • Both Setaria yehi and Rumenfilaria andersoni were detected by both diagnostic testing approaches.

Cite This Study

Kulpa et al. (2026) studied this question.

synapsesocial.com/papers/69e9b71b85696592c86eb185https://doi.org/10.1017/s0031182026101991
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Using deep amplicon sequencing as a molecular xenomonitoring approach for detecting filarial nematodes in biting arthropod vectors2025
  2. 2Elucidating nematode diversity and prevalence in moose across a wide latitudinal gradient using DNA metabarcoding2024 · 2 citations
  3. 3Early-life exposure to vector-borne pathogens in moose calves from Finland: molecular and phylogenetic evidence2026
  4. 4Health Status and Drivers of Endoparasite Infection in a Low-Density Moose (Alces alces) Population.2026
  5. 5Hepatic Morphological Lesions and Blood Biochemical Evidence of Impaired Metabolism in Wild European Moose ( <i>Alces alces</i> ) Naturally Infected With <i>Parafasciolopsis fasciolaemorpha</i>2026