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April 23, 2026Acta Crystallographica Section D Structural BiologyOpen Access

Mapping the effects of specific radiation damage and solvent radiolysis in buffers and crystals with online UV–Vis absorption spectroscopy

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Authors

JSJack StubbsNCNicolas CaramelloMRMatthew J. Rodrigues

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Overview

Online UV-Vis absorption spectroscopy maps X-ray-induced spectral changes in buffers and crystals, suggesting improved data interpretation.

Key Points

  • The study aims to map the effects of radiation damage and solvent radiolysis on buffers and crystals using UV-Vis absorption spectroscopy.
  • Utilized online UV-Vis absorption spectroscopy during crystallographic data collection.
  • Investigated spectral changes in common crystallization chemicals and mixtures at cryogenic temperatures.
  • Analyzed the chromophoric enzyme intermediate I320 in vitamin B6 biosynthesis.
  • Detected X-ray-induced spectral changes at doses as low as 1 kGy.
  • Observed a transient increase in absorption between 450 and 700 nm due to solvated electron absorption.
  • Identified distinct absorption peaks for halides in buffers.

Cite This Study

Stubbs et al. (2026) studied this question.

synapsesocial.com/papers/69e9b85585696592c86eb9f7https://doi.org/10.1107/s2059798326002743
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