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April 23, 2026Veterinary Sciences0 citationsOpen Access

Genetic Diversity and Population Structure Analysis of Seven Duck Populations of Bangladesh Using Microsatellite Markers

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PSPC SahaKBKrishna Chandra BarmanMKM N Kim

Key Points

  • This research aims to analyze the genetic diversity, population structure, and phylogenetic relationships of duck populations in Bangladesh.
  • Used 14 microsatellite markers to assess genetic diversity among seven duck populations.
  • Evaluated genetic measures like allele frequency, heterozygosity, and Shannon’s index across individuals.
  • Conducted phylogenetic analysis and population differentiation using F-statistics.
  • Identified a total of 133 alleles with a mean of 9.50 alleles per locus.
  • Moderate genetic diversity was found, with 75% of diversity within populations and 16% among populations.
  • Indigenous populations clustered distinctly from crossbreds and Pekin ducks in phylogenetic analysis.

Abstract

The objectives of this paper were to assess the genetic diversity, population structure, genetic differentiation, and phylogenetic relationships among seven duck populations using 14 microsatellite (MS) markers. This paper included 176 individuals representing seven duck populations of Bangladesh: indigenous duck (BLD), Nageswari (NAG), Rupali (RUP), Jinding (JIN), Pekin (PEK), BAU Black and White (BWC), and BAU White (WHC). A total of 133 alleles were observed with a mean of 9.50 alleles per locus. Genetic diversity was evaluated using measures such as allele frequency, observed and expected heterozygosity, and Shannon’s information index with average values of 5.44 ± 0.31, 0.59 ± 0.02, 0.64 ± 0.02, and 1.28 ± 0.05, respectively. Population differentiation and inbreeding analysis (F-statistics) indicated moderate genetic diversity and a slight degree of inbreeding across populations. Analysis of molecular variance indicated that 75% of the total genetic diversity was attributable to the within-population variation, whereas 9% and 16% were attributed to the variation among individuals and population differentiation, respectively. Indigenous duck populations (BLD, NAG, and RUP) had a close genetic relationship with JIN ducks and an intermediate relationship with two crossbreds (BWC and WHC), and the highest genetic distance was observed with PEK ducks. Neighbor-joining phylogenetic analysis revealed that the Bangladeshi indigenous duck populations formed a single cluster, while the two crossbreds (BWC and WHC) and PEK exhibited their distinct genetic identities in separate clusters. Furthermore, structure analysis at K = 2 to 5 confirmed the distinct genetic architecture (ΔK = 4.00) of the studied duck populations. This paper provides important insights into genetic diversity measures and population differentiation that will be helpful in future genetic improvement, conservation initiatives, and the design of appropriate breeding programs.

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Cite This Study

Saha et al. (2026) studied this question.

synapsesocial.com/papers/69e9b89b85696592c86ebb7chttps://doi.org/10.3390/vetsci13040407
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