A Dot-Array Magnetic Enrichment Strategy for Rapid, Multiplexed Detection of Viable Bacteria via ATP Bioluminescence

Puntos clave

• The aim is to develop a method for rapid and sensitive detection of viable bacteria using ATP bioluminescence.
• Utilized a dot-array magnetic enrichment strategy to isolate bacteria.
• Applied ATP bioluminescence for detection in complex herbal medicine samples.
• Evaluated method performance in the presence of matrix interference.
• Achieved significant reduction in interference from complex samples.
• Demonstrated effective detection of low-abundance bacteria.
• Enabled multiplexed analysis, allowing simultaneous detection of multiple bacterial species.