Aging increases susceptibility to a wide range of diseases, partially due to alterations in T lymphocytes. This study aimed to characterize age-related changes in T-cell output, phenotype, and function under basal conditions and after myocardial infarction (MI). Compared with young mice (2-6 months), aged mice (≥18 months) demonstrated reduced thymic size and fewer developing thymocytes. Accordingly, circulating T-cell counts were significantly lower in aged mice than in young mice. Interestingly, the spleen and bone marrow of aged mice showed increased T-cell accumulation, primarily due to expansion of memory T cells. Similarly, older humans (≥60 years old) exhibited reduced circulating T cells and a higher proportion of memory T cells. Under basal conditions, aged splenic T cells expressed higher mRNA levels of pro-inflammatory and cytotoxic factors (e.g. Ifnγ, Tnfα, granzyme b, and perforin). Moreover, aged T cells exhibited a higher frequency of PD-1 + , TIGIT + , LAG-3 + , and CTLA-4 + cells compared to young T cells, indicative of an exhausted phenotype. Metabolically, aged T cells showed higher basal glycolytic and mitochondrial oxidative phosphorylation. Following MI, thymic regeneration was evident in young mice by day 14, whereas the aged thymus remained atrophic. Additionally, aged MI mice exhibited reduced naïve T cells and increased memory T cells in the spleen and bone marrow than young mice. In conclusion, our study reveals that aging diminishes thymocyte output and circulating T-cell pools, facilitates memory T-cell accumulation, and compromises thymic regeneration under basal conditions and post-MI.
Konovalova et al. (Fri,) studied this question.