Astrocytic Cytoskeletal Infrastructure Constrains Large-Scale Glial Signaling Dynamics · Cytoszkieletowa infrastruktura astrocytów ogranicza dynamikę wielkoskalowej sygnalizacji glejowej

Bilingual Preprint (EN ∥ PL) Astrocytes form a brain-wide, gap-junction-coupled network that supports spatially extended calcium signaling and modulates neuronal activity. We propose that astrocytic cytoskeletal architecture — particularly microtubule-dependent transport and morphology — defines a set of biophysical constraints that shape the accessible space of glial signaling dynamics. Three experimentally grounded mechanisms are synthesized: (i) cytoskeletal maintenance of astrocyte morphology and spatial coverage; (ii) microtubule-based transport of organelles and signaling machinery required for calcium-wave propagation; and (iii) cytoskeleton-dependent trafficking and stabilization of connexin-based gap junctions. The framework yields testable predictions linking cytoskeletal integrity to emergent network behavior, is supported by a minimal reaction–diffusion model, and provides a multiscale bridge between intracellular organization and large-scale glial dynamics.