What question did this study set out to answer?

The study aims to isolate and evaluate the antioxidant potential of resveratrol from grape seeds, particularly focusing on its bioactive properties from local varieties.

April 26, 2026Recent trends in pharmacology.1 citationsOpen Access

Isolation, NMR Characterization, and Comparative In Vitro Antioxidant Evaluation of Resveratrol from Grape Seeds (Vitis vinifera L.)

NKNisagül Kaya SŞSamed Şimşek SGSefa Gözcü

Key Points

The study aims to isolate and evaluate the antioxidant potential of resveratrol from grape seeds, particularly focusing on its bioactive properties from local varieties.
500 g of grape seeds extracted with methanol via reflux for 4 hours
Isolated trans-resveratrol using Sephadex LH-20 and confirmed via NMR spectroscopy
Antioxidant capacity assessed through DPPH and ABTS scavenging assays, compared to standards
Yielded 10 mg of pure trans-resveratrol after purification
Trans-resveratrol showed DPPH IC₅₀ of 17.33 ± 1.22 µg/mL
ABTS IC₅₀ for trans-resveratrol was 6.84 ± 0.47 µg/mL, outperforming Trolox and ascorbic acid.

Abstract

Objective: The present study aimed to isolate, characterize, and evaluate the in vitro antioxidant potential of resveratrol from grape seeds (Vitis vinifera L.) obtained from local markets in Erzincan, Türkiye. It is hypothesized that the unique microclimate of that region enhances the bioactive profile of local grape varieties, making them a significant source of natural antioxidants.Methods: Grape seeds (500 g) were extracted using 100% methanol via reflux for 4 hours (three replicates). The crude extract (30 g) underwent liquid-liquid extraction with ethyl acetate to yield a concentrated phenolic fraction (2 g). For isolation, Sephadex LH-20 adsorbent was used as the stationary phase and eluted with methanol. Structural characterization was conducted using 1H and 13C NMR spectroscopy. The antioxidant capacity of the isolated resveratrol was determined through DPPH and ABTS radical scavenging assays, comparing results with standard antioxidants (Trolox, BHA, BHT, and Ascorbic acid).Results: Chromatographic purification yielded 10 mg of pure trans-resveratrol. The structure was firmly verified by NMR spectroscopy, with the high olefinic coupling constant (J = 16.3 Hz) suggesting the trans structure. In antioxidant experiments, Trans-resveratrol demonstrated notable DPPH radical scavenging action (IC₅₀ = 17.33 ± 1.22 µg/mL). Trans-resveratrol outperformed Trolox (7.22 ± 0.24 µg/mL) and ascorbic acid (8.22 ± 0.37 µg/mL) in the ABTS test, with an IC₅₀ of 6.84 ± 0.47 µg/mL.Conclusion: The findings demonstrate that grape seeds from Erzincan markets are an excellent source of highly bioactive trans-resveratrol. The isolated compound exhibited exceptional antioxidant efficiency, particularly in the ABTS assay where it surpassed several commercial standards. These results suggest that local grape varieties possess significant potential as natural sources for high-purity bioactive agents in the nutraceutical and pharmaceutical sectors.

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