Investigation of storage time dependence on cytoprotective effect by pressurized dissolution of argon gas in cold storage

The storage time dependence on protective effect by pressurized dissolution of argon gas in cold storage on a rat heart striated myocytes monolayer were investigated. The cell monolayers incubated in a culture dish for 24 h were prepared as test samples. The samples were pressurized in 0 and 0.8 MPa in argon gas, and then preserved at 6°C for 0 to 72 h. The pressure-dependent cell viability was evaluated using water-soluble tetrazolium salts assay. The results showed that protective effect in after 24 h was low without pressure, but it was high with pressure. The protective effects in storage time of 48 h and 72 h were low even with pressurized dissolution of argon gas, but they were slightly higher than that without it.