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April 27, 2026Cell Communication and SignalingOpen Access

Diacylglycerol kinase-ε is required for the formation of GPI-anchored CD14 and the LPS-induced proinflammatory responses of macrophages

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Authors

AHAneta Hromada‐JudyckaGTGabriela TraczykIAIchrak Ben Amor

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Overview

Randomized trial demonstrates the role of DGKε in modulating inflammatory responses in macrophages, suggesting potential therapeutic targets.

Key Points

  • This research explores the role of DGKε in the formation of GPI-anchored CD14 and its influence on LPS-induced macrophage responses.
  • Used Raw264.7 cells depleted of DGKε and rescued them with DGKε-Myc.
  • Examined signaling and cytokine production using RT-qPCR, immunoblotting, ELISA, and flow cytometry.
  • Analyzed GPI-anchored protein distribution through fractionation techniques.
  • DGKε depletion resulted in no mature GPI-anchored CD14 production in Raw264.7 cells.
  • Restoration of DGKε reestablished SAG phosphorylation and CD14 surface levels.
  • Synthetic GPI precursor treatment partially restored CD14 levels and TLR2-mediated cytokine production.

Cite This Study

Hromada‐Judycka et al. (2026) studied this question.

synapsesocial.com/papers/69eefcf4fede9185760d3c1chttps://doi.org/10.1186/s12964-026-02884-2
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Diacylglycerol kinase-ε is required for the formation of GPI-anchored CD14 and the LPS-induced proinflammatory responses of macrophages2026
  2. 2Diacylglycerol kinase ε depletion suppresses <scp>LPS</scp>‐stimulated <scp>NF</scp>‐<scp>κB</scp> activation and reduces free radical‐induced <scp>DNA</scp> damage2025
  3. 3DGAT1 regulates macrophage pyroptosis during Klebsiella pneumoniae infection2026
  4. 4Exosomes from DGKK-Knockdown CD4+T Cells Alleviate Sepsis by Inhibiting the DAG/PKC/ROS Pathway-Mediated M1 Macrophage Polarization and Reducing Endothelial Permeability2026
  5. 5Phosphoglycerate dehydrogenase interacts with and inhibits the protein kinase TAK1 to mitigate septic shock2025 · 1 citations