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April 27, 2026npj VaccinesOpen Access

Prefusion-stabilized SARS-CoV-2 spike reshapes antigenic hierarchy and antibody targeting against conserved and occluded epitopes

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Authors

SOShintaro OishiRKRyutaro KotakiHDHisham M. Dokainish

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Overview

Randomized trial shows reduced neutralizing potency in vaccine recipients compared to convalescents, suggesting changes in antibody targeting.

Key Points

  • This research aims to understand how the structure of the SARS-CoV-2 spike protein affects antibody responses in vaccinated individuals compared to those recovered from infection.
  • Evaluated neutralizing antibody responses in large cohorts of vaccinated and convalescent individuals.
  • Measured the neutralizing potency index (NPI) and RBD IgG titers.
  • Utilized molecular dynamics simulations and binding assays to study epitope exposure.
  • NPI is approximately fivefold lower in vaccine recipients compared to convalescent individuals.
  • The lower NPI is linked to greater targeting of conserved and occluded RBD epitopes.
  • Stabilizing mutations in the vaccine spike antigen alter conformational dynamics, exposing occluded epitopes.

Cite This Study

Oishi et al. (2026) studied this question.

synapsesocial.com/papers/69eefd82fede9185760d4324https://doi.org/10.1038/s41541-026-01464-2
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