This research evaluates the use of high-field solid-state NMR to characterize ganglioside GD3-catalyzed Aβ42 aggregates, focusing on overcoming hurdles posed by their heterogeneity.
Utilized 1.1 GHz solid-state NMR to study GD3-catalyzed Aβ42 aggregates.
Analyzed uniformly-labeled Aβ42 incubated with GD3 under magic-angle spinning conditions.
Conducted 13C CPMAS spectra and 2D 13C-13C chemical shift correlation experiments using CORD mixing.
1.1 GHz spectra showed enhanced sensitivity and improved spectral resolution compared to 600 MHz.
Better resolved resonances corresponded to selectively structured regions of Aβ42.
Presence of an ordered core within the lipid-associated aggregates was indicated.