PulseExploreJournal ClubResearchersJournals
Instagram
HomeJournal ClubExplore
Synapse
⌘+K
Synapse
April 29, 2026ACS Infectious Diseases

Rational Design and Screening of Chemically Modified Anti-SARS-CoV-2 siRNA

View Full Paper
Ask AI
Bookmark
Share

Authors

ZCZihui ChengYCYing CaiEZEnhua Zhang

Discussion

Loading...

Member takes

Overview

Screening identified lead compound VS-38m, suggesting chemical modifications enhance siRNA stability and reduce cytotoxicity.

Key Points

  • To enhance RNA interference against SARS-CoV-2 by optimizing siRNA through chemical modifications.
  • Screened 200 siRNAs targeting RNA-dependent RNA polymerase gene.
  • Evaluated modification patterns with 2'-fluoro and 2'-O-methyl substitutions.
  • Incorporated various 3'-end modifications, including glycol and threose nucleic acids.
  • Identified VS-38m as a lead compound among screened siRNAs.
  • Optimized siRNA VS-159m102 showed superior gene-silencing efficacy at picomolar concentrations.
  • Improvements included reduced cytotoxicity and better serum stability.

Cite This Study

Cheng et al. (2026) studied this question.

synapsesocial.com/papers/69f154e0879cb923c4945343https://doi.org/10.1021/acsinfecdis.6c00095
View Full Paper
Ask AI
Bookmark
Share