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April 29, 2026Journal of Agricultural and Food Chemistry1 citations

Directed Evolution of Glucosyltransferase ZmDo and Its Application in the Synthesis of Luteolin-5-O-Xyloside

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JXJiaqi XuCLChangning LuLYLei Yao

Key Points

  • To improve the glucosyltransferase ZmDo enzyme for better synthesis of luteolin-5-O-xyloside.
  • Engineered glucosyltransferase ZmDo through directed evolution.
  • Constructed a de novo UDP-Xyl biosynthetic pathway for cofactor regeneration.
  • Optimized conditions to maximize product yield and conversion rate.
  • Achieved a titer of 4451 mg/L of luteolin-5-O-xyloside.
  • Obtained an 87% conversion rate in optimized conditions.
  • Enhanced enzyme activity was 2.3-fold compared to the wild type.

Abstract

), 2.3-fold higher than the wild type. A de novo UDP-Xyl biosynthetic pathway was constructed, enabling intracellular cofactor regeneration. Under optimized conditions, a titer of 4451 mg/L with an 87% conversion rate was achieved.

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Cite This Study

Xu et al. (2026) studied this question.

synapsesocial.com/papers/69f19f74edf4b4682480643ahttps://doi.org/10.1021/acs.jafc.6c01223
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