This research demonstrates metabolic rewiring to improve itaconate yield in Escherichia coli through CO2 fixation, indicating potential for microbial production enhancement.
Key Points
To improve itaconate yield through metabolic rewiring and CO2 fixation in Escherichia coli.
Reengineering central carbon metabolism in Escherichia coli
Blocking pyruvate oxidation to acetyl-CoA
Promoting CO2 incorporation into TCA cycle intermediates
Utilizing glucose and acetate co-metabolism
Achieved an itaconate yield of 0.81 C mol/C substrate mol
Yield nears theoretical maximum from glucose of 0.83 C mol/C mol
Demonstrated direct incorporation of CO2 into itaconate production