The aim is to quantify ATP levels in cells using HPLC techniques.
HPLC analysis of ATP standard and cellular ATP
Use of Poroshell 120 EC-C18 column
Chromatograms compared for standard and cellular samples.
ATP content was successfully quantified in cellular samples through HPLC.
The chromatograms provided clear distinction between ATP standard and cellular levels.
Abstract
The overlap HPLC chromatograms of ATP standard (red) and ATP in cells (blue). Poroshell 120 EC-C18 (3 × 150 mm, 2.7 μm), 25°C, pH 6.8 buffer solution of 0.05 mol/L KH