The proteins represent one of the most commercially significant groups of enzymes because of their extensive usages in various industries. This combined research is devoted to the production, characterization, and industrial role of the protease enzymes with a focus on their functional adaptability and biochemical diversity. Microorganisms that produce proteases were culture is isolated and screened of various environmental sources then optimized conditions in order to increase enzyme yield. pH, temperature, substrate concentration, incubation time was put to systematic evaluation to optimize production efficiency. Protease obtained was purified by common biochemical methods and then determined by gram staining and then described in terms of its molecular weight, ideal activity parameters and stability characteristics. The enzyme exhibited active over a wide pH and temperature range, which makes the application in different possible industrial processes. Kinetic analysis also indicated its catalytic activity and substrate specificity. The article places importance on the industrial application of proteases in the detergent industry among other sectors Such as leather processing, food and dairy production, pharmaceuticals and waste management. Their environmentally friendly and their capacity to substitute harsh chemical working methods highlight the fact that they are increasingly becoming significant in environmentally friendly industrial practices. Overall, this research leads to the knowledge on protease production and functionality, providing information on their improved use in industrial processes and biotechnology.
Ashu Husain1, Mohammad Saad2, Giriraj Tripathi3, Jyoti Kumari8, Abhishank Gangwar3, Bhoomika4, Amber Singh Kushwah5, Muskan Kumari6, Krati Upadhayaya7, Shobha Rajput7, Vinod Kumar Gupta8* (2026) studied this question.